{"id":2819,"date":"2022-09-26T13:17:53","date_gmt":"2022-09-26T16:17:53","guid":{"rendered":"https:\/\/sites.usp.br\/cryoemschool2023\/?page_id=2819"},"modified":"2023-07-04T17:08:19","modified_gmt":"2023-07-04T20:08:19","slug":"topics-and-program","status":"publish","type":"page","link":"https:\/\/sites.usp.br\/cryoemschool2023\/topics-and-program\/","title":{"rendered":"Topics and Program"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"2819\" class=\"elementor elementor-2819\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-cb70fb1 elementor-section-stretched elementor-section-content-middle elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"cb70fb1\" data-element_type=\"section\" data-settings=\"{&quot;stretch_section&quot;:&quot;section-stretched&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ebb4766\" data-id=\"ebb4766\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4bf37c4 elementor-widget elementor-widget-heading\" data-id=\"4bf37c4\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-xl\">Topics and Program<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2286337 elementor-button-info elementor-align-left elementor-widget elementor-widget-button\" data-id=\"2286337\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-md\" href=\"https:\/\/drive.google.com\/file\/d\/1PA7bS20Au5JNHielCaBCzJTzYiko82yX\/view?usp=sharing\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Final Program \u2014 Access here!<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d3683f8 elementor-widget elementor-widget-text-editor\" data-id=\"d3683f8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>The S\u00e3o Paulo School of Advanced Science in CryoEM aims to cover the theoretical and practical foundations of advanced CryoEM techniques used to determine macromolecular structures. Time will be divided between lectures, demonstrations and hands-on practical sessions given by internationally renowned researchers in the field of CryoEM.<\/p><p>Topics that will be covered include sample preparation, data collection, processing of high-resolution image data, single particle analysis, in-cell structural biology by cryo electron tomography (CryoET), model building and model validation. Invited and participating researchers will also present their most recent results.<\/p><p>A detailed program will be made available after participant selection. A preliminary list of topics can be found below:<\/p><p style=\"padding-left: 40px;\">\u2022 Historical perspective of single particle EM and CryoEM<br \/>\u2022 Electron microscope architecture<br \/>\u2022 Detectors and Motion Correction<br \/>\u2022 Sample Preparation and Vitrification<br \/>\u2022 Symmetry<br \/>\u2022 Fourier Transforms<br \/>\u2022 Cryo-EM data collection<br \/>\u2022 Contrast Transfer Function<br \/>\u2022 Masks and Filters<br \/>\u2022 2D and 3D classification<br \/>\u2022 Conformational Heterogeneity<br \/>\u2022 Membrane proteins (sample preparation, data collection and processing)<br \/>\u2022 Cryo-electron tomography, subtomogram averaging and in-cell structural<br \/>\u2022 Atomic Model Building<br \/>\u2022 Helical reconstruction<br \/>\u2022 Validation of Models and Maps<br \/>\u2022 Data deposition and Databases<br \/>\u2022 Practical Sessions in Single Particle Analysis and subtomogram averaging<br \/>\u2022 Oral presentations from participating PIs illustrating the application of CryoEM and CryoET methods to investigate biological system<br \/>\u2022 Invited student oral presentations<br \/>\u2022 Poster sessions\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Topics and Program Final Program \u2014 Access here! The S\u00e3o Paulo School of Advanced Science in CryoEM aims to cover the theoretical and practical foundations of advanced CryoEM techniques used to determine macromolecular structures. Time will be divided between lectures, demonstrations and hands-on practical sessions given by internationally renowned researchers in the field of CryoEM. 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