Energy and Fitness
Measurement of energy expenditure and activity using Oxymax/CLAMS. Oxymax/CLAMS (Columbus Instruments Comprehensive Lab Animal Monitoring System) metabolic cages are used to simultaneously measure metabolic rate (MR) represented by energy expenditure, physical activity and indirect calorimetry in laboratory animals. The experiment non-invasively measures O² consumption and CO² production in individual mice using metabolic chambers and calculates the respiratory exchange ratio to reflect energy expenditure.
Redox & Bioenergetics. The Seahorse XF-96 analyzer provides measurements of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of living cells in a 96-well plate format. Measures the rate of change of analytes dissolved oxygen and pH in media immediately surrounding living cells cultured in a microplate. Changes in the extracellular media are caused by the consumption or production of analytes by the cells. Therefore, a sensitive measurement of the media flux can be used to determine rates of cellular metabolism with great precision and in a totally noninvasive, label free manner.