{"id":1426,"date":"2025-06-30T15:00:52","date_gmt":"2025-06-30T17:00:52","guid":{"rendered":"https:\/\/sites.usp.br\/neuroreplab\/?page_id=1426"},"modified":"2025-06-30T18:20:51","modified_gmt":"2025-06-30T20:20:51","slug":"overview","status":"publish","type":"page","link":"https:\/\/sites.usp.br\/neuroreplab\/en\/overview\/","title":{"rendered":"Overview"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"1426\" class=\"elementor elementor-1426\" data-elementor-post-type=\"page\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c3f1918 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c3f1918\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b9aaeef\" data-id=\"b9aaeef\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-edffb72 elementor-widget elementor-widget-heading\" data-id=\"edffb72\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Research<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ada00ff elementor-widget elementor-widget-text-editor\" data-id=\"ada00ff\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Our research group has been dedicated to studying the physiology of neurons that synthesize kisspeptins, a peptide essential for the onset of puberty and regulation of reproductive functions.<\/p><p>We investigate how different factors, including stress related to food intake, influence the development and electrical activity of these neurons.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-5f083a9 e-con-full e-flex e-con e-parent\" data-id=\"5f083a9\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-5df328d elementor-skin-carousel elementor-arrows-yes elementor-pagination-type-bullets elementor-pagination-position-outside elementor-widget elementor-widget-media-carousel\" data-id=\"5df328d\" data-element_type=\"widget\" data-settings=\"{&quot;slides_per_view&quot;:&quot;1&quot;,&quot;skin&quot;:&quot;carousel&quot;,&quot;effect&quot;:&quot;slide&quot;,&quot;show_arrows&quot;:&quot;yes&quot;,&quot;pagination&quot;:&quot;bullets&quot;,&quot;speed&quot;:500,&quot;autoplay&quot;:&quot;yes&quot;,&quot;autoplay_speed&quot;:5000,&quot;loop&quot;:&quot;yes&quot;,&quot;pause_on_hover&quot;:&quot;yes&quot;,&quot;pause_on_interaction&quot;:&quot;yes&quot;,&quot;space_between&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;space_between_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]},&quot;space_between_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:10,&quot;sizes&quot;:[]}}\" data-widget_type=\"media-carousel.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-swiper\">\n\t\t\t<div class=\"elementor-main-swiper swiper\">\n\t\t\t\t<div class=\"swiper-wrapper\">\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"swiper-slide\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-carousel-image\" role=\"img\" aria-label=\"34-06-2024-F-KG-NOlit-AVPV-ARH\" style=\"background-image: url(&#039;https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/04\/34-06-2024-F-KG-NOlit-AVPV-ARH.png&#039;)\">\n\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t<div class=\"swiper-slide\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-carousel-image\" role=\"img\" aria-label=\"20210729_104809\" style=\"background-image: url(&#039;https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/04\/20210729_104809-scaled.jpg&#039;)\">\n\n\t\t\t\n\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"swiper-pagination\"><\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<div class=\"elementor-swiper-button elementor-swiper-button-prev\" role=\"button\" tabindex=\"0\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"eicon-chevron-left\"><\/i>\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Anterior<\/span>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<div class=\"elementor-swiper-button elementor-swiper-button-next\" role=\"button\" tabindex=\"0\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"eicon-chevron-right\"><\/i>\t\t\t\t\t\t\t<span class=\"elementor-screen-only\">Pr\u00f3ximo<\/span>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8657c9b elementor-widget__width-inherit elementor-widget elementor-widget-video\" data-id=\"8657c9b\" data-element_type=\"widget\" data-settings=\"{&quot;video_type&quot;:&quot;hosted&quot;,&quot;autoplay&quot;:&quot;yes&quot;,&quot;controls&quot;:&quot;yes&quot;}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"e-hosted-video elementor-wrapper elementor-open-inline\">\n\t\t\t\t\t<video class=\"elementor-video\" src=\"https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/04\/3.17.2-adicionar-esse-video-ao-item-nossa-pesquisa.mp4\" autoplay=\"\" controls=\"\" controlsList=\"nodownload\"><\/video>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-ec27e0e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"ec27e0e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-02a03f0\" data-id=\"02a03f0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2c17c45 elementor-widget elementor-widget-heading\" data-id=\"2c17c45\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Main Publications<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-a7d2140 e-flex e-con-boxed e-con e-parent\" data-id=\"a7d2140\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-77c8a45 e-con-full e-flex e-con e-child\" data-id=\"77c8a45\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-586f79c elementor-widget elementor-widget-heading\" data-id=\"586f79c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Mansano NS; Vieira HR; Araujo-Lopes R; Szwka RE; Donato J Jr; Frazao R. Fasting modulates GABAergic synaptic transmission to arcuate kisspeptin neurons in female mice.<b> Endocrinology<\/b> (FI: 3.8), v. 164 (11), 2023.<\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-7f6aafa e-con-full e-flex e-con e-child\" data-id=\"7f6aafa\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2e56c3c elementor-widget elementor-widget-heading\" data-id=\"2e56c3c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Mansano NS; Paradela RS; Bohlen TM; Zanardi IM; Chaves FM; Silveira MA; Tavares MR; Donato J Jr; Frazao R. Vasoactive intestinal peptide exerts an excitatory effect on hypothalamic kisspeptin neurons during estrogen negative feedback. Molecular and cellular end<b>ocrinology<\/b> (FI: 4,1), v. 542, 2022.<\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-320c967 e-con-full e-flex e-con e-child\" data-id=\"320c967\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-7e389c0 elementor-widget elementor-widget-heading\" data-id=\"7e389c0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Bohlen TM; Zampieri TT; Furigo EC; Teixeira PDS; List EO; Kopchick; Donato J Jr; Frazao R. Central growth hormone signaling contributes to the timing of puberty in female mice. <b>Journal of Endocrinology<\/b> (FI: 4,3), v. 243(3): 161-173, 2019.<\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-6a08f1a e-con-full e-flex e-con e-child\" data-id=\"6a08f1a\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-3f03bfe elementor-widget elementor-widget-heading\" data-id=\"3f03bfe\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Paula DG; Bohlen TM; Tessaria TT; Mansano NS; Vieira HR; Gusmao DO; Wasinski, F; Donato J Jr; Frazao R. Distinct effects of growth hormone deficiency and disruption of hypothalamic kisspeptin system on reproduction of male mice. <b>Life Sciences<\/b> (FI: 5,04), v. 285 (2021) 119970. <\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-c55792e e-con-full e-flex e-con e-child\" data-id=\"c55792e\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-64548b6 elementor-widget elementor-widget-heading\" data-id=\"64548b6\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\">Frazao R; Cravo, R, M; Donato, J Jr; Clegg, D; Elmquist, J; Zigman, J, M; Williams, K, W; Eias, C, F. Shift in Kiss1 cell activity requires estrogen receptor \u03b1. <b>J. Neuroscience<\/b> (FI: 4.4), v14(3):301-312, 2013<\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-272c8c3 e-con-full e-flex e-con e-child\" data-id=\"272c8c3\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-4439e00 elementor-widget elementor-widget-heading\" data-id=\"4439e00\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=frazao+R&#038;sort=date%20\"><p data-start=\"1139\" data-end=\"1187\">Check out more publications from the group:<\/p><\/a><\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3225f19 elementor-widget elementor-widget-heading\" data-id=\"3225f19\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h6 class=\"elementor-heading-title elementor-size-default\"><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/?sort=date&#038;term=frazao+R&#038;sort_order=desc\">https:\/\/pubmed.ncbi.nlm.nih.gov\n\n\n<\/a><\/h6>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-25665ee elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"25665ee\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-78cc82c\" data-id=\"78cc82c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-412a263 elementor-widget elementor-widget-heading\" data-id=\"412a263\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Methods Used<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9d2b3d4 elementor-widget elementor-widget-spacer\" data-id=\"9d2b3d4\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t<div class=\"elementor-element elementor-element-43f2b43 e-flex e-con-boxed e-con e-parent\" data-id=\"43f2b43\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-cf8ecb0 e-con-full e-flex e-con e-child\" data-id=\"cf8ecb0\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-909c419 elementor-widget elementor-widget-image\" data-id=\"909c419\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"2560\" height=\"2560\" src=\"https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-scaled.jpg\" class=\"attachment-full size-full wp-image-1156\" alt=\"\" srcset=\"https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-scaled.jpg 2560w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-300x300.jpg 300w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-1024x1024.jpg 1024w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-150x150.jpg 150w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-768x768.jpg 768w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-1536x1536.jpg 1536w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-2048x2048.jpg 2048w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-600x600.jpg 600w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-45x45.jpg 45w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-200x200.jpg 200w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/Estro-400x400.jpg 400w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/06\/elementor\/thumbs\/Estro-scaled-r6xaiiklbm5qcrinjpudxssbwqil4uwlwsfufsebea.jpg 4w\" sizes=\"(max-width: 2560px) 100vw, 2560px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-8dc4825 elementor-widget elementor-widget-heading\" data-id=\"8dc4825\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">In vivo evaluation (sexual maturation and estrous cycle)<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-3eb9b45 e-con-full e-flex e-con e-child\" data-id=\"3eb9b45\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-b54032c elementor-widget elementor-widget-image\" data-id=\"b54032c\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/app.jove.com\/t\/64989\/hypothalamic-kisspeptin-neurons-as-target-for-whole-cell-patch-clamp\">\n\t\t\t\t\t\t\t<img decoding=\"async\" width=\"1040\" height=\"1392\" src=\"https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/02\/WhatsApp-Image-2025-02-09-at-16.00.42.jpeg\" class=\"attachment-full size-full wp-image-410\" alt=\"\" srcset=\"https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/02\/WhatsApp-Image-2025-02-09-at-16.00.42.jpeg 1040w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/02\/WhatsApp-Image-2025-02-09-at-16.00.42-224x300.jpeg 224w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/02\/WhatsApp-Image-2025-02-09-at-16.00.42-765x1024.jpeg 765w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/02\/WhatsApp-Image-2025-02-09-at-16.00.42-768x1028.jpeg 768w, https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/02\/WhatsApp-Image-2025-02-09-at-16.00.42-400x535.jpeg 400w\" sizes=\"(max-width: 1040px) 100vw, 1040px\" \/>\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a127957 elementor-widget elementor-widget-heading\" data-id=\"a127957\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Electrophysiology: Whole-cell patch-clamp: study of hypothalamic neuron activity<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-2898dcb e-con-full e-flex e-con e-child\" data-id=\"2898dcb\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-e3764fa elementor-widget elementor-widget-heading\" data-id=\"e3764fa\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Calcium imaging: identification of factors\/hormones that modulate hypothalamic neuron activity<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-fd3735b e-flex e-con-boxed e-con e-parent\" data-id=\"fd3735b\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-d8a633a e-flex e-con-boxed e-con e-parent\" data-id=\"d8a633a\" data-element_type=\"container\">\n\t\t\t\t\t<div class=\"e-con-inner\">\n\t\t<div class=\"elementor-element elementor-element-bc8fb5d e-con-full e-flex e-con e-child\" data-id=\"bc8fb5d\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-8e2fae7 elementor-widget elementor-widget-text-editor\" data-id=\"8e2fae7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ul><li>Histology<\/li><li>RNA and DNA extraction<\/li><li><p data-start=\"1967\" data-end=\"2002\">Real-time and quantitative PCR<\/p><\/li><li><p data-start=\"1967\" data-end=\"2002\"><span style=\"font-weight: 400;\">Conventional PCR<\/span><\/p><\/li><li>Genotyping<\/li><li><p data-start=\"2161\" data-end=\"2194\">Genetically modified animals<\/p><\/li><\/ul>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t<div class=\"elementor-element elementor-element-93c539e e-con-full e-flex e-con e-child\" data-id=\"93c539e\" data-element_type=\"container\">\n\t\t\t\t<div class=\"elementor-element elementor-element-2555f1e elementor-widget elementor-widget-text-editor\" data-id=\"2555f1e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p data-start=\"2323\" data-end=\"2411\">All procedures are previously approved by the Ethics Committee on Animal Use (CEUA) <strong>link:<\/strong><b><a href=\"https:\/\/ww3.icb.usp.br\/ceua\/\">https:\/\/ww3.icb.usp.br\/ceua\/<\/a><\/b><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Research Our research group has been dedicated to studying the physiology of neurons that synthesize kisspeptins, a peptide essential for the onset of puberty and regulation of reproductive functions. We investigate how different factors, including stress related to food intake, influence the development and electrical activity of these neurons. Previous Next https:\/\/sites.usp.br\/neuroreplab\/wp-content\/uploads\/sites\/1629\/2025\/04\/3.17.2-adicionar-esse-video-ao-item-nossa-pesquisa.mp4 Main Publications Mansano [&hellip;]<\/p>\n","protected":false},"author":24540,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-1426","page","type-page","status-publish","hentry","entry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.5 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Overview - Neurofisiologia da Reprodu\u00e7\u00e3o<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/sites.usp.br\/neuroreplab\/en\/overview\/\" \/>\n<meta name=\"twitter:label1\" content=\"Est. tempo de leitura\" \/>\n\t<meta name=\"twitter:data1\" content=\"2 minutos\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/sites.usp.br\\\/neuroreplab\\\/en\\\/overview\\\/\",\"url\":\"https:\\\/\\\/sites.usp.br\\\/neuroreplab\\\/en\\\/overview\\\/\",\"name\":\"Overview - 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