{"id":250,"date":"2020-09-09T10:15:12","date_gmt":"2020-09-09T12:15:12","guid":{"rendered":"http:\/\/sites.usp.br\/ppggastro\/?page_id=250"},"modified":"2020-09-09T10:15:12","modified_gmt":"2020-09-09T12:15:12","slug":"mgt5723","status":"publish","type":"page","link":"https:\/\/sites.usp.br\/ppggastro\/mgt5723\/","title":{"rendered":"MGT5723"},"content":{"rendered":"

Relat\u00f3rio de Dados da Disciplina<\/h1>\n

\u00a0<\/strong><\/p>\n

Sigla:\u00a0 MGT5723 – 9\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Tipo: POS<\/p>\n

Nome: Modelos Experimentais e Metodologia Cient\u00edfica em Doen\u00e7as Hep\u00e1ticas e Gastrointestinais<\/p>\n

\u00c1rea: Ci\u00eancias em Gastroenterologia (5168)<\/p>\n

 <\/p>\n

Datas de aprova\u00e7\u00e3o:<\/p>\n

CCP: 11\/10\/2019\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 CPG: 13\/02\/2020\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 CoPGr:<\/p>\n

Data de ativa\u00e7\u00e3o: 13\/02\/2020\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Data de desativa\u00e7\u00e3o:<\/p>\n

 <\/p>\n

Carga hor\u00e1ria:<\/p>\n

Total: 60 h\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Te\u00f3rica: 5 h\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Pr\u00e1tica: 16 h\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Estudo: 9 h<\/p>\n

Cr\u00e9ditos: 4\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Dura\u00e7\u00e3o: 2 Semanas<\/p>\n

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 <\/p>\n

 <\/p>\n

 <\/p>\n

Respons\u00e1veis:<\/p>\n

207293 – Estela Regina Ramos Figueira – 13\/02\/2020 at\u00e9 data atual 420180 – Eleazar Chaib – 13\/02\/2020 at\u00e9 data atual<\/p>\n

977511 – Fl\u00e1vio Henrique Ferreira Galvao – 13\/02\/2020 at\u00e9 data atual<\/p>\n

2182898 – Claudia Pinto Marques Souza de Oliveira – 13\/02\/2020 at\u00e9 data atual 5843775 – Alberto Luiz Monteiro Meyer – 13\/02\/2020 at\u00e9 data atual<\/p>\n

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 <\/p>\n

Objetivos:<\/p>\n

    \n
  1. Aprofundar os conhecimentos de metodologia cient\u00edfica aplicada em modelos experimentais em afec\u00e7\u00f5es do aparelho digestivo.<\/li>\n
  2. Introduzir e aprimorar a vis\u00e3o cr\u00edtica ao aluno de p\u00f3s-gradua\u00e7\u00e3o sobre elabora\u00e7\u00e3o de projetos de pesquisa experimental visando sua aplicabilidade cl\u00ednica.<\/li>\n
  3. Introduzir aos alunos de p\u00f3s-gradua\u00e7\u00e3o a vis\u00e3o \u00e9tica e de biosseguran\u00e7a em projetos que envolvam animais de pequeno e grande porte.<\/li>\n
  4. Capacitar os alunos de p\u00f3s-gradua\u00e7\u00e3o para entendimento dos conceitos e fundamentos b\u00e1sicos dos modelos experimentais de transplante de \u00f3rg\u00e3os, doen\u00e7as hep\u00e1ticas, pancre\u00e1ticas e gastrointestinais.<\/li>\n
  5. Capacitar o aluno na aplica\u00e7\u00e3o de modelos experimentais para o desenvolvimento de projetos de pesquisa b\u00e1sica\/translacional<\/li>\n<\/ol>\n

     <\/p>\n

     <\/p>\n

    Justificativa:<\/p>\n

    Nos \u00faltimos anos, houve expans\u00e3o acentuada do conhecimento das doen\u00e7as hep\u00e1ticas e no campo dos transplantes de \u00f3rg\u00e3os e c\u00e9lulas, notadamente no f\u00edgado, p\u00e2ncreas, intestinos, multivisceral, ilhotas pancre\u00e1ticas, hepat\u00f3citos e c\u00e9lulas tronco.<\/p>\n

    O transplante de \u00f3rg\u00e3os \u00e9 procedimento de alta complexidade, com grande potencial para pesquisa cient\u00edfica e ainda com v\u00e1rias limita\u00e7\u00f5es relacionadas, a falta de doadores, limita\u00e7\u00e3o do tempo de preserva\u00e7\u00e3o dos enxertos, doen\u00e7as linfoproliferativas, problemas relacionados a rejei\u00e7\u00e3o, doen\u00e7a enxerto versus hospedeiro, recidiva da doen\u00e7a e problemas relacionados \u00e0 imunosupress\u00e3o. Estas complica\u00e7\u00f5es justificam pesquisas no \u00e2mbito experimental que se ocupem em desenvolver novos modelos de tratamento menos agressivos e mais eficazes. Por outro lado, houve tamb\u00e9m um grande avan\u00e7o no estudo da fisiopatog\u00eanese e terap\u00eautica de algumas afec\u00e7\u00f5es hep\u00e1ticas como a Doen\u00e7a Hep\u00e1tica Gordurosa N\u00e3o Alco\u00f3lica e o Carcinoma Hepatocelular, incluindo conhecimento da biologia molecular na progress\u00e3o destas doen\u00e7as, podendo-se pesquisar alvos moleculares que tenham aplicabilidade terap\u00eautica.<\/p>\n

    Paralelamente a estes modelos imp\u00f5e-se o ensino da metodologia de pesquisa cient\u00edfica aplicada a realidade cl\u00ednica. A formula\u00e7\u00e3o de hip\u00f3teses cient\u00edficas vem acompanhada do conhecimento cient\u00edfico adquirido na pr\u00e1tica cl\u00ednica e experimental, ela deve seguir, se poss\u00edvel, sequ\u00eancia metodol\u00f3gica bem estabelecida: inicialmente em modelos n\u00e3o biol\u00f3gicos (matem\u00e1ticos e virtuais). Se a hip\u00f3tese, for positiva deve-se iniciar modelos em vitro com utiliza\u00e7\u00e3o de cultura de c\u00e9lulas e tecidos. Em sequ\u00eancia a pesquisa deve passar para modelos em animais de pequeno porte (roedores), para posteriormente utilizar animais de grande porte. O laborat\u00f3rio de pesquisa portanto \u00e9 passo fundamental para elabora\u00e7\u00e3o de hip\u00f3teses e projetos de pesquisa para ensinamento de metodologia cient\u00edfica visando-se o aprimoramento m\u00e9dico e aplicabilidade cl\u00ednica.<\/p>\n

     <\/p>\n

    Conte\u00fado:<\/p>\n

    AULAS TE\u00d3RICAS ( 05x 2h= 10hs)<\/p>\n

    AULAS PR\u00c1TICAS (16 hs semanais= 32hs) ESTUDOS E SEMIN\u00c1RIOS (9 hs semanais= 18hs)<\/p>\n

     <\/p>\n

    Metodologia Cient\u00edfica e Bio\u00e9tica em Pesquisa Aplica\u00e7\u00f5es de Novos Conhecimentos em Medicina Banco de Dados em Pesquisa<\/p>\n

    Modelos experimentais em transplante Modelos experimentais em c\u00e9lulas e tecidos<\/p>\n

    Modelos experimentais em animal de pequeno porte<\/p>\n

    Mecanismos de Carcinog\u00eanese Hep\u00e1tica e Modelos Experimentais de CHC Modelos experimentais de Pancreatite Aguda<\/p>\n

    Modelos experimentais de Doen\u00e7a Hep\u00e1tica Gordurosa N\u00e3o Alco\u00f3lica Modelos experimentais de neoplasias malignas do trato gastrointestinal Modelos experimentais da Doen\u00e7a Inflamat\u00f3ria Intestinal Demonstra\u00e7\u00e3o pr\u00e1tica dos Modelos Experimentais<\/p>\n

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    Bibliografia:<\/p>\n

    Figueira ERR, Rocha-Filho JA, Lanchotte C, Coelho AMM, Nakatani M, Tatebe ER, Lima JAV, Mendes CO, de Araujo BCRP, Abdo EE, D’Albuquerque LC, Galv\u00e3o FHF. Sevoflurane Preconditioning plus Postconditioning Decreases Inflammatory Response with Hemodynamic Recovery in Experimental Liver Ischemia Reperfusion. Gastroenterol Res Pract. 2019:5758984. eCollection<\/p>\n

    de Mesquita GHA, Jardim YJ, Iuamoto LR, Suguita FY, Essu FF, Oliveira LT, Meyer A, Crescenzi A, Rocha-Santos V, Galv\u00e3o FHF, Andraus W, Chaib E, D’Albuquerque LAC. Gastric fundus submucosa as a site for islets transplantation: An experimental study. Ann Med Surg (Lond). 2018;28:1-5.<\/p>\n

    Abdo EE, Figueira ERR, Rocha-Filho JA, Chaib E, D’Albuquerque LAC, Bacchella T. Preliminary results of topical hepatic hypothermia in a model of liver ischemia\/reperfusion injury in rats. Arq Gastroenterol. 2017;54(3):246-249.<\/p>\n

    Iuamoto LR, Franco AS, Suguita FY, Essu FF, Oliveira LT, Kato JM, Torsani MB, Meyer A, Andraus W, Chaib E, D’Albuquerque LAC. Human islet xenotransplantation in rodents: A literature review of experimental model trends. Clinics (Sao Paulo). 2017;72(4):238-243.<\/p>\n

    Tiburcio TC, Willebrords J, da Silva TC, Pereira IV, Nogueira MS, Crespo Yanguas S, Maes M, Silva ED, Dagli ML, de Castro IA, Oliveira CP, Vinken M, Cogliati B. Connexin32 deficiency is associated with liver injury, inflammation and oxidative stress in experimental non-alcoholic steatohepatitis. Clin Exp Pharmacol Physiol. 2017;44(2):197-206.<\/p>\n

    Galv\u00e3o FH, Waisberg DR, Seid VE, Costa AC, Chaib E, Baptista RR, Capelozzi VL, Lanchotte C, Cruz RJ, Araki J, D’Albuquerque LC. Allogeneic anorectal transplantation in rats: technical considerations and preliminary results. Sci Rep. 2016;6:30894.<\/p>\n

    Vivoli E, Cappon A, Milani S, Piombanti B, Provenzano A, Novo E, Masi A, Navari N, Narducci R, Mannaioni G, Moneti G, Oliveira CP, Parola M, Marra F. NLRP3 inflammasome as a target of berberine in experimental murine liver injury: interference with P2X7 signalling. Clin Sci (Lond). 2016;130(20):1793-806.<\/p>\n

    Rocha-Santos V, Figueira ER, Rocha-Filho JA, Coelho AM, Pinheiro RS, Bacchella T, Machado MC, D’Albuquerque LA. Pentoxifylline enhances the protective effects of hypertonic saline solution on liver ischemia reperfusion injury through inhibition of oxidative stress. Hepatobiliary Pancreat Dis Int. 2015;14(2):194-200.<\/p>\n

    Seid VE, Galv\u00e3o FH, Vaidya A, Waisberg DR, Cruz RJ Jr, Chaib E, Nahas SC, Araujo SE, D’Albuquerque LA, Araki J.Functional outcome of autologous anorectal transplantation in an experimental model. Br J Surg. 2015;102(5):558-62. Canedo BF, Galvao FH, Ducatti L, Nacif LS, Catanozi S, Soler WV, Chaib E, D’Albuquerque LA, Andraus W. Liver Autotransplantation in Pigs without Venovenous Bypass: A Simplified Model using a Supraceliac Aorta Cross-Clamping Maneuver. Ann Transplant. 2015;20:320-6.<\/p>\n

    Evangelista FS, Muller CR, Stefano JT, Torres MM, Muntanelli BR, Simon D, Alvares-da-Silva MR, Pereira IV, Cogliati B, Carrilho FJ, Oliveira CP. Physical training improves body weight and energy balance but does not protect against hepatic steatosis in obese mice. Int J Clin Exp Med. 2015;8(7):10911-9.<\/p>\n

    Figueira ER, Rocha-Filho JA, Nakatani M, Buto MF, Tatebe ER, Andre VO, Cecconello I, D’Albuquerque LA. Hepatic ischemic preconditioning increases portal vein flow in experimental liver ischemia reperfusion injury. Hepatobiliary Pancreat Dis Int. 2014;13(1):40-7.<\/p>\n

    van der Heijden KM, van der Heijden IM, Galvao FH, Lopes CG, Costa SF, Abdala E, D’Albuquerque LA, Levin AS. Intestinal translocation of clinical isolates of vancomycin-resistant Enterococcus faecalis and ESBL-producing Escherichia coli in a rat model of bacterial colonization and liver ischemia\/reperfusion injury. PLoS One. 2014;9(9):e108453.<\/p>\n

    Iuamoto LR, Meyer A, Chaib E, D’Albuquerque LA. Review of experimental attempts of islet allotransplantation in rodents: parameters involved and viability of the procedure. World J Gastroenterol. 2014 Oct 7;20(37):13512-20. doi: 10.3748\/wjg.v20.i37.13512.<\/p>\n

    Meyer A, Kubrusly MS, Salemi VM, De Mendon\u00e7a Coelho AM, Molan NA, Patzina RA, Machado MC, Mady C,<\/p>\n

    D’Albuquerque LA, Jukemura J. Severe acute pancreatitis: a possible role of intramyocardial cytokine production. JOP. 2014 May 27;15(3):237-42.<\/p>\n

    Raposo Amaral AS, Pawlick RL, Rodrigues E, Pepper A, Galv\u00e3o FH, Correa-Giannella ML, Shapiro AM. Glutathione Ethyl Ester Supplementation during Pancreatic Islet Isolation Improves Viability and Transplant Outcomes in a Murine Marginal Islet Mass Model. PLoS One. 2013; 8(2):e552-88.<\/p>\n

    Galv\u00e3o FH, Waisberg DR, Cruz RJ Jr, Chaib E, Carneiro D’Albuquerque LA. Modified multivisceral transplantation in the rat. Transplantation. 2013;96(2):e3-4.<\/p>\n

    Galvao FH, Soler W, Pompeu E, Waisberg DR, Mello ES, Costa AC, Teodoro W, Velosa AP, Capelozzi VL, Antonangelo L, Catanozi S, Martins A, Malbouisson LM, Cruz RJ Jr, Figueira ER, Filho JA, Chaib E, D’Albuquerque LA. Immunoglobulin G profile in hyperacute rejection after multivisceral xenotransplantation. Xenotransplantation.<\/p>\n

    2012;19(5):298-304.<\/p>\n

    Galvao FH, Seid VE, Waisberg DR, Cruz RJ Jr, Hirano H, Catanozi S, Chaib E, D’Albuquerque LA. An innovative model of autologous anorectal transplantation with pudendal nerve reconstruction. Clinics. 2012; 67(8):971-2.<\/p>\n

    Marques TG, Chaib E, da Fonseca JH, Louren\u00e7o AC, Silva FD, Ribeiro MA Jr, Galv\u00e3o FH, D’Albuquerque LA. Review of experimental models for inducing hepatic cirrhosis by bile duct ligation and carbon tetrachloride injection. Acta Cir Bras. 2012; 27(8):589-94.<\/p>\n

    Galv\u00e3o FH, Waisberg DR, De Mello Vianna RM, De Castro Galv\u00e3o R, Seid VE, Andraus W, Chaib E, D’Albuquerque LA. Intestinal transplantation including anorectal segment in the rat. Microsurgery. 2012; 32(1):77-9<\/p>\n

    Waisberg DR, Lee AD, Santos RM, Mory EK, Costa AL, Montero EF, Chaib E, D’Albuquerque LA, Galvao FH. Small bowel transplantation in outbred rats. Acta Cir Bras. 2011; 26(6):496-502.<\/p>\n

    Waisberg DR, Galv\u00e3o FH, De Castro Galv\u00e3o R, Chaib E, D’Albuquerque LA.Intestinal transplantation using cuff-glue sutureless technique for microanastomosis in rats. Microsurgery. 2011; 31(7):584-5.<\/p>\n

    Stefano JT, Cogliati B, Santos F, Lima VM, Mazo DC, Matte U, Alvares-da-Silva MR, Silveira TR, Carrilho FJ, Oliveira CP. S-Nitroso-N-acetylcysteine induces de-differentiation of activated hepatic stellate cells and promotes antifibrotic effects in vitro. Nitric Oxide. 2011 30;25(3):360-5.<\/p>\n

    Chaib E, Galv\u00e3o FH, Rocha-Filho JA, Silveira BL, Chen L, de C Carvalho FS, Waisberg DR, de Souza YE, Machado MC, D’Albuquerque LA. Total pancreatectomy: porcine model for inducing diabetes – anatomical assessment and surgical aspects. Eur Surg Res. 2011;46(1):52-5.<\/p>\n

    Figueira ER, Bacchella T, Coelho AM, Sampietre SN, Molan NA, Leit\u00e3o RM, Machado MC. Timing-dependent protection of hypertonic saline solution administration in experimental liver ischemia\/reperfusion injury. Surgery. 2010;147(3):415-23. de Lima VM, Oliveira CP, Alves VA, Chammas MC, Oliveira EP, Stefano JT, de Mello ES, Cerri GG, Carrilho FJ, Caldwell SH. A rodent model of NASH with cirrhosis, oval cell proliferation and hepatocellular carcinoma. J Hepatol. 2008; 49(6):1055-61.<\/p>\n

      \n
    1. Oliveira CP, de Lima VM, Simplicio FI, Soriano FG, de Mello ES, de Souza HP, Alves VA, Laurindo FR, Carrilho FJ, de Oliveira MG Prevention and reversion of nonalcoholic steatohepatitis in OB\/OB mice by S-nitroso-N-acetylcysteine treatment. J Am Coll Nutr. 2008;27(2):299-305.<\/li>\n
    2. Lima VMR, Oliveira CPMS, Sawada LY, Barbeiro HV, Mello ES, Soriano FG, Alves VAF, Caldwell SH, Carrilho FJ. Yo Jyo Hen Shi Ko (YHK), A Novel Chinese Herbal, Prevents Nonalcoholic Steatohepatitis in Ob\/Ob Mice Fed a High Fat or Methionine-Choline Deficient Diet. Liver International. 2007;27:227-34.<\/li>\n<\/ol>\n

      Oliveira CPMS, Alves VAF, Lima VMR, Stefano JT, Debbas V, Sa SV, Wakamatsu A, Correa-Gianella ML, Mello ES, Tiniakos D, Oliveira MG, Gianella-Neto D, Caldwell SH, Laurindo FRM, Carrilho FJ. Modulation of Hepatic Microsomal Trigliceride Transfer Protein Induced by S-Nitroso-N-Acetylciste\u00edne in Ob\/Ob Mice. Biochemical Pharmacology. 2007;74: 290-297<\/p>\n

      Stefano JT, Oliveira CPMS, Correa-Giannella ML, Lima VMR, S\u00e1 SV, Oliveira EP, Mello ES, Giannella-Neto D, Alves VAF, Carrilho FJ. Nonalcoholic Steatohepatitis (NASH) in Ob\/Ob Mice Treated with Yo Jyo Hen Shi Ko (YHK): Effects on Peroxisome Proliferator-Activated Receptors (PPARs) and Microsomal Triglyceride Transfer Protein (MTTP). Digestive Diseases and Sciences. 2007;52(12):3448-54.<\/p>\n

      Fo\u00ebx BA. – The ethics of animal experimentation. Emerg Med J. 2007;24(11): 750 751.<\/p>\n

      Galv\u00e3o FH, de Andrade J\u00fanior DR, de Andrade DR, Martins BC, Marson AG, Bernard CV, Dos Santos SA, Bacchella T, Machado MC. Hepatocyte Transplantation: State of the Art. Hepatology Research. 2006;36(4):237-47.<\/p>\n

      Machado MA, Galv\u00e3o FH, Pompeu E, Ribeiro C, Bacchella T, Machado MC. A canine model of laparoscopic segmental liver resection. J Laparoendosc Adv Surg Tech A. 2004;14(5):325-8.<\/p>\n

       <\/p>\n

       <\/p>\n

      Forma de avalia\u00e7\u00e3o:<\/p>\n

      Vide campo Observa\u00e7\u00e3o.<\/p>\n

       <\/p>\n

       <\/p>\n

      Observac\u00e3o:<\/p>\n

      CRIT\u00c9RIOS DE AVALIA\u00c7\u00c3O:<\/p>\n

      A avalia\u00e7\u00e3o ser\u00e1 feita por nota de participa\u00e7\u00e3o, frequ\u00eancia e escrita de um projeto que use modelo animal. A m\u00e9dia destas avalia\u00e7\u00f5es ser\u00e1 a nota final atribu\u00edda pela Disciplina.","protected":false},"excerpt":{"rendered":"

      Relat\u00f3rio de Dados da Disciplina \u00a0 Sigla:\u00a0 MGT5723 – 9\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Tipo: POS Nome: Modelos Experimentais e Metodologia Cient\u00edfica em Doen\u00e7as Hep\u00e1ticas e Gastrointestinais \u00c1rea: Ci\u00eancias em Gastroenterologia (5168)   Datas de aprova\u00e7\u00e3o: CCP: 11\/10\/2019\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 CPG: 13\/02\/2020\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 CoPGr: Data de ativa\u00e7\u00e3o: 13\/02\/2020\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Data de desativa\u00e7\u00e3o:   Carga hor\u00e1ria: Total: 60 h\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Te\u00f3rica: 5 h\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Pr\u00e1tica: 16 […]<\/p>\n","protected":false},"author":21987,"featured_media":0,"parent":0,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-250","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/pages\/250","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/users\/21987"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/comments?post=250"}],"version-history":[{"count":2,"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/pages\/250\/revisions"}],"predecessor-version":[{"id":252,"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/pages\/250\/revisions\/252"}],"wp:attachment":[{"href":"https:\/\/sites.usp.br\/ppggastro\/wp-json\/wp\/v2\/media?parent=250"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}