ACH5528 Frontiers in Biotechnology II

Diego Antonio Falceta Gonçalves (dfalceta@usp.br)

Eutimio Gustavo Fernández Núñez (egfnunez@usp.br)

Felipe Santiago Chambergo Alcalde (fscha@usp.br)

Grzegorz Kowal (grzegorz.kowal@usp.br)

Patrícia Targon Campana (pcampana@usp.br)

Tânia Araújo Viel (taniaviel@usp.br)

Viviane Abreu Nunes Cerqueira Dantas (vanunes@ib.usp.br)

6 credits (9ECTS): 4 (theoretical classes) + 2 (field trip)

 Lecture schedule

Tuesday, 14:00-18:00 h - Building I1 – Room: 135

Course description

The course will provide an overview of advanced topics of several sciences such as computing science, fluid biophysics, cell therapy and tissue bioengineering, neuroscience and bioprocess engineering, which represent important fields of interface and interaction opportunities with Biotechnology. The knowledge built in this discipline should favor the performance in the area of Biotechnology, opening perspectives for the student to identify potential applications of the topics dealt with in situations of professional practice and with the development of products and services of biotechnological interest.

Program

THEORETICAL PART

Brazilian Biodiversity

Pharmaceutics and Tissue bioengineering

  • Nanomaterials
  • Stem cells and cell therapy
  • Biomaterials and 3D cultures

Neuroscience

  • Neurotransmission, synapses and neuroplasticity
  • Central nervous system modeling for the study of brain aging and neurodegenerative diseases

Fluid Biophysics

  • Basics of fluid dynamics
  • Applications to the cardiovascular system
  • Air dynamics and respiration

Data Science

  • Data mining
  • Bioinformatics
  • Modeling of complex systems

Bioprocess engineering

  • Principles of the upstream and downstream stages for manufacturing biomolecules holding commercial interest
  • Experimentation throughout the development stages of a bioprocess
  • Economic assessment of technological alternatives

PRACTICAL PART (FIELD TRIP)

Analysis of problems in complex situations of the Brazilian reality in excursions to critical regions that allow highlighting crucial demands of the contemporary world.

 Evaluation

Students will be evaluated on the basis of a 20 min oral presentation (previously prepared either individually or in a group) on a topic chosen by the student themselves. The following criteria will be considered in the assessment: clarity of presentation, organization of content, amplitude and precision of notions

 Attendance and Participation

Attendance is mandatory in 70% of the activities.

 Grading and approval criteria

Grade equal to or greater than 5.0: student approved;

Grade between 3.0 and 4.9: requires a 2nd evaluation step (test to be taken in January);

Grade below 3.0: failure.

References

Tissue bioengineering

  1. Nourian Dehkordi, A. et al. Skin tissue engineering: wound healing based on stem-cell-based therapeutic strategies. Stem Cell Res Ther, v. 10, n. 1, p. 111, 2019.
  2. Chua, A. W. et al. Skin tissue engineering advances in severe burns: review and therapeutic applications. Burns Trauma, v. 19, n. 4, p. 3, 2016.
  3. Yi, S. et al. (2016). Extracellular Matrix Scaffolds for Tissue Engineering and Regenerative Medicine. Curr Stem Cell Res Ther.2016 Sep 4.[Epub ahead of print].
  4. Muzzio, N., Moya, S., Romero, G. Multifunctional scaffolds and synergistic strategies in tissue engineering and regenerative medicine. v. 13, n. 6, 2021. DOI: 10.3390/pharmaceutics13060792.
  5. Di Marzio, N., Eglin, D., Serra, T., et al. Bio-Fabrication: Convergence of 3D Bioprinting and Nano-Biomaterials in Tissue Engineering and Regenerative Medicine. Frontiers in Bioengineering and Biotechnology. v. 8, n. April, 2020. DOI: 10.3389/fbioe.2020.00326.
  6. Lin, K., Zhang, D., Macedo, M. H., et al. Advanced Collagen-Based Biomaterials for Regenerative Biomedicine. Advanced Functional Materials v. 29, n. 3, 2019. DOI: 10.1002/adfm.201804943.
  7. Hernandez, E. D. D., Reyes-Romero, J. R. Materials for Biomedical Engineering Thermoset and Thermoplastic Polymer, Chapter 14: Characteristics of polymeric materials used in medicine. [S.l.], Elsevier Inc., 2019. v. 1. Disponível em: http://dx.doi.org/10.1016/B978-0-12-816874-5.00014-1.
  8. Yilmaz, B., Al Rashid, A., Mou, Y. A., et al. Bioprinting: A review of processes, materials and applications. v. 23, n. April, p. e00148, 2021. DOI: 10.1016/j.bprint.2021.e00148. Disponível em: https://doi.org/10.1016/j.bprint.2021.e00148.
  9. Steffens, D. et al. Development of a biomaterial associated with mesenchymal stem cells and keratinocytes for use as a skin substitute. Regen Med, v. 10, n. 8, p. 975-987, 2015.
  10. Xie, S.Y. et al. Adult stem cells Seeded on electrospinning silk fibroin nanofibrous scaffold enhance wound repair and regeneration. J Nanosci Nanotechnol, v. 16, n. 6, p. 5498-5505, 2016.

 Fluid biophysics

  1. H Badeeer, Hemodynamics For Medical Students, Advances In Physiology Education, 2001.
  2. Joachim D Pleil et al. The physics of human breathing: flow, timing, volume, and pressure parameters for normal, on-demand, and ventilator respiration J. Breath Res. 15 042002, 2021.

 Data science

  1. Oliveira, A.L. (2019), Biotechnology, Big Data and Artificial Intelligence. Biotechnol. J., 14: 1800613. https://doi.org/10.1002/biot.201800613.
  2. Moore JH. Empowering the data science scientist. BioData Min. 2021 Jan 23;14(1):8. doi: 10.1186/s13040-021-00246-x. PMID: 33485343; PMCID: PMC7827983.
  3. Persidis, A. Data mining in biotechnology. Nat Biotechnol 18, 237–238 (2000). https://doi.org/10.1038/72722.
  4. Harline K, Martínez-Gómez J, Specht CD and Roeder AHK (2021) A Life Cycle for Modeling Biology at Different Scales. Front. Plant Sci. 12:710590. doi: 10.3389/fpls.2021.710590.

Bioprocess engineering

  1. Cornell, J.A. Experiments with Mixtures: Designs, Models, and the Analysis of Mixture Data. 3rd. ed. New York, NY: John Wiley & Sons, Inc., 2002.
  2. COUPER, J.R. Process Engineering Economics. 1st ed. New York: CRC Press, 2003.
  3. Montgomery, D.C. Design and Analysis of Experiments. 10th. ed. Hoboken, NJ, USA: Wiley, 2020.
  4. Shuler, M.L.; Kargi, F.; Delisa, M. Bioprocess Engineering: Basic Concepts. Third ed. Upper Saddle River: Prentice Hall, 2017.

 

 

TURMAS

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