Título:Professor Titular
Laboratório: Laboratório de Biologia Celular e Molecular
Telefone: +55 (11) 3091-7362
E-mail: gpam@usp.br
Lattes: http://lattes.cnpq.br/3033400086575581
Equipe:
Pós-doutorado:
Jackeline Soares de Oliveira Beltran (jackbeltran13@gmail.com)
Doutorado:
Tandressa S. Berguetti (tata.berguetti@usp.br)
Samson Kosemani (kosemanisamson2014@gmail.com)
Abolaji Samson Olagunju (olagunjuabolajisamson@gmail.com)
Mestrado:
Ronaldo Rodrigues Ribeiro (ronaldo.ribeiro@usp.br)
Marcela Bittar Araujo Lima (marcela.lima@usp.br)
Andrew Victor Diniz Sardinha (andrewsardinha@icb.usp.br)
Iniciação Científica:
Raiane Silva de Morais (raianemorais@usp.br)
Linha de Pesquisa:
Controle molecular da morte celular no câncer e no sistema imune.
Resumo:
Gustavo P. Amarante-Mendes é Professor Titular do Instituto de Ciências Biomédicas da Universidade de São Paulo (ICB-USP), onde foi Vice-Diretor de 2017-2021. Foi Segundo Secretário (2004-2005), Vice-Presidente (2012-2013) e Presidente (2016-2017) da Sociedade Brasileira de Imunologia, e Vice-Presidente da Federação das Sociedades de Biologia Experimental (FESBE) (2017-2021). Foi Coordenador do Programa de Pós-graduação em Imunologia (2011-2015) e Presidente da Comissão Interna de Biossegurança (2009-2013) do ICB-USP. Possui graduação em Ciências Biológicas Modalidade Médica pela Universidade de Santo Amaro (1984), mestrado (1989), doutorado (1993) e Livre-docência (2009) em Imunologia pela Universidade de São Paulo. Foi Research Associate no La Jolla Institute for Allergy and Immunology (San Diego, CA) e Visiting Scientist no Department of Genetics, Trinity College Dublin e na Genentech, Inc, uma empresa do grupo Roche. É membro do corpo editorial da Cell Death and Disease, uma revista do grupo NATURE, da Frontiers in Cell and Developmental Biology, faz parte do Editorial Advisory Panel da Biochemical Journal e é um dos Keystone Symposia Programming Consultants, desde 2012. Recebeu várias premiações científicas, entre elas o 2o Prêmio Oncologia Novartis – Saúde Brasil, Nature Review Cancer Award em 2007 (aluno de doutorado Daniel Diniz de Carvalho), Menção Honrosa no Prêmio CAPES de Tese em 2009 (aluno Ricardo Weinlich) e Prêmio CAPES de Tese em 2010 (aluno Daniel Diniz de Carvalho). Organizou diversos congressos nacionais e internacionais, incluindo os dois primeiros Keystone Symposia da América Latina, em 2013 e 2014. Publicou cerca de 130 trabalhos científicos, os quais, em conjunto, apresentam mais do que 3.500 citações. Tem experiência nas áreas de Imunologia, Biologia Celular e Oncologia Experimental, atuando principalmente nos seguintes temas: morte celular, apoptose, piroptose, necroptose, imunoregulação, oncogenes, leucemia mieloide crônica, melanoma.
Principais Trabalhos
- DNA hypomethylating agents increase activation and cytolytic activity of CD8+ T cells. Loo Yau H, Bell E, Ettayebi I, de Almeida FC, Boukhaled GM, Shen SY, Allard D, Morancho B, Marhon SA, Ishak CA, Gonzaga IM, da Silva Medina T, Singhania R, Chakravarthy A, Chen R, Mehdipour P, Pommey S, Klein C, Amarante-Mendes GP, Roulois D, Arribas J, Stagg J, Brooks DG, De Carvalho DD. Mol Cell. 2021 Apr 1;81(7):1469-1483.e8. doi: 10.1016/j.molcel.2021.01.038. Epub 2021 Feb 19. PMID: 33609448
- Absence of Bim sensitizes mice to experimental Trypanosoma cruzi infection. Hernández-Torres M, Silva do Nascimento R, Rebouças MC, Cassado A, Matteucci KC, D’Império-Lima MR, Vasconcelos JRC, Bortoluci KR, Alvarez JM, Amarante-Mendes GP. Cell Death Dis. 2021 Jul 10;12(7):692. doi: 10.1038/s41419-021-03964-6.
- RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes. Rana A, de Almeida FC, Paico Montero HA, Gonzales Carazas MM, Bortoluci KR, Sad S, Amarante-Mendes GP. Front Immunol. 2020 Apr 9;11:536. doi: 10.3389/fimmu.2020.00536. eCollection 2020. PMID: 32328060
- Pattern Recognition Receptors and the Host Cell Death Molecular Machinery. Amarante-Mendes GP, Adjemian S, Branco LM, Zanetti LC, Weinlich R, Bortoluci KR. Front Immunol. 2018 Oct 16;9:2379. doi: 10.3389/fimmu.2018.02379. eCollection 2018. PMID: 30459758
- Buzzo CL, Medina T, Branco LM, Lage SL, Ferreira LC, Amarante-Mendes GP, Hottiger MO, De Carvalho DD, Bortoluci KR. Epigenetic regulation of nitric oxide synthase 2, inducible (Nos2) by NLRC4 inflammasomes involves PARP1 cleavage. Sci Rep. 7:41686, (2017).
- Amarante-Mendes GP, Griffith TS. Therapeutic applications of TRAIL receptor agonists in cancer and beyond. Pharmacol Ther. 155:117-31, (2015).
- De Carvalho DD, Mello BP, Pereira WO & Amarante-Mendes GP. PRAME/EZH2-mediatedregulation of TRAIL: a new target for câncer therapy. Curr. Mol. Med. 13:296-304 (2013).
- Clemente T, Dominguez MR, Vieira NJ, Rodrigues MM & Amarante-Mendes GP. In vivo assessment of specific cytotoxic T lymphocyte killing. Methods 61:105-109 (2013).
- Lage SL, Buzzo CL, Amaral EP, Matteucci KC, Massis LM, Icimoto MY, Carmona AK, D’Império Lima MR, Rodrigues MM, Ferreira LC, Amarante-Mendes GP, Bortoluci KR. Cytosolic flagellin-induced lysosomal pathway regulates inflammasome-dependent and -independent macrophage responses. Proc Natl Acad Sci USA. 27;110(35):E3321-30, (2013).
- Vasconcelos JR, Bruña-Romero O, Araújo AF, Dominguez MR, Ersching J, de Alencar BC, Machado AV, Gazzinelli RT, Bortoluci KR, Amarante-Mendes GP, Lopes MF, Rodrigues MM. Pathogen-Induced Proapoptotic Phenotype and High CD95 (Fas) Expression Accompany a Suboptimal CD8(+) T-Cell Response: Reversal by Adenoviral Vaccine. PLoS Pathog., 8:e1002699, (2012).
- Pereira WO, Amarante-Mendes GP. Apoptosis: a program of cell death or cell disposal? Scand J Immunol. 73:401-407, (2011).
- De Carvalho DD, Binato R, Pereira WO, Leroy JMG, Colassanti MD, Proto-Siqueira R, Bueno-da-Silva AEB, Zago MA, Zanichelli MA, Abdelhay E, Castro FA, Jacysyn JF, Amarante-Mendes GP. BCR-ABL-mediated up-regulation of PRAME is responsible for knocking down TRAIL in CML patients. Oncogene, 30:223-233, (2011).
- Pedrosa AMC, Weinlich R, Mognol GP, Robbs BK, Viola JPB, Campa A, Amarante-Mendes GP. Melatonin protects CD4+ T cells from activation-induced cell death by blocking NFAT-mediated CD95L upregulation. J. Immunol., 184:3487-3494, (2010).
- Weinlich R, Bortoluci KR, Chehab CF, Serezani CH, Ulbrich AG, Peters-Golden M, Russo M, Amarante-Mendes GP. TLR4/MYD88-Dependent, LPS-induced synthesis of PGE2 by macrophages or dendritic cells prevents anti -CD3-mediated CD95L upregulation in T cells. Cell Death Diff., 15:1901-1909, (2008).
- Tanjoni I, Weinlich R, Della-Casa MS, Clissa PB, Saldanha-Gama RF, Freitas MS, Barja-Fidalgo C, Amarante-Mendes GP, Moura-da-Silva AM. Jararhagin, a snake venom toxin, induces a specialized form of apoptosis (anoikis) selective to endothelial cells. Apoptosis, 10: 851-861, (2005).
- Brumatti G, Weinlich R, Chehab CF, Yon M, Amarante-Mendes GP. Comparison of the anti-apoptotic effects of Bcr-Abl, Bcl-2 and Bcl-xL following diverse apoptogenic stimuli. FEBS Lett., 541:57-63, (2003).
- Bueno-da Silva AEB, Brumatti G, Russo FO, Green DR, Amarante-Mendes GP. Bcr-Abl-mediated resistance to apoptosis is independent of constant tyrosine-kinase activity. Cell Death Diff. 5: 592-598, (2003).
- Muotri AR, Marchetto MCN, Suzuki MF, Okazaki K, Lotfi CFP, Brumatti G, Amarante-Mendes GP, Menck CFM. Low amounts of the DNA repair XPA protein are sufficient to recover UV-resistance. Carcinogenesis 23:1039-1046, (2002).
- De Freitas Balanco JM, Moreira MEC, Bonomo A, Bozza PT, Amarante-Mendes GP, Pirmez C, Barcinski M. Apoptotic mimicry by an obligate intracellular parasite down regulates macrophage microbicidal activity. Curr. Biol., 11: 1870-1873, (2001).
- Wolf BB, Goldstein JC, Stennicke HR, Beere H, Amarante-Mendes GP, Salvesen G, Green DR. Calpain functions in a caspase-independent manner to promote apoptosis-like events during platelet activation. Blood, 94:1683-1692, (1999).
- Amarante-Mendes GP, Kim CN, Liu L, Huang Y, Perkins CL, Green DR, Bhalla K. Bcr-Abl exerts its anti-apoptotic effect against diverse apoptotic stimuli through blockage of mitochondrial release of cytochrome c and activation of caspase-3. Blood, 91: 1700-1705, (1998).
- Amarante-Mendes GP, McGahon AJ, Nishioka WK, Afar DEH, Witte ON, Green DR. Bcl-2-independent Bcr-Abl-mediated resistance to apoptosis: Protection is correlated with up regulation of Bcl-xL. Oncogene, 16: 1383-1390, (1998).
- Amarante-Mendes GP, Finucane DM, Martin SJ, Cotter TG, Green DR. Anti-apoptotic oncogenes prevent caspase-dependent and independent cell death. Cell Death Diff., 5:298-306, (1998).
- Martin SJ, Amarante-Mendes GP, Shi L, Chuang T-H, Casiano CA, O’Brien GA, Fitzgerald P, Tan EM, Bokoch GM, Greenberg AH, Green DR. The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism. EMBO J., 15:2407-2416, (1996).
- Martin SJ, Finucane DM, Amarante-Mendes GP, O’Brien GA, Green DR. Phosphatidylserine externalization during CD95-induced apoptosis of both cells and cytoplasts requires ICE/CED-3 protease activity. J. Biol. Chem., 271: 28753-28756, (1996).