Profa. Dra. Alessandra Pontillo

Título: Professor Associado

Laboratório: Imunogenética

Telefone: +55 (11) 3091 7367

E-mail: alepontillo@usp.br

Lattes: http://lattes.cnpq.br/5264956586383796

ORCID: 0000-0002-7883-0640

Lab Website: https://alepontillo.wixsite.com/immunogeneticsicbusp

Linhas de Pesquisa

Genética do inflamassoma
Caracterização do inflamassoma em modelos in vitro

Interesses e projetos

A investigação da “genética do inflamassoma” abre novos horizontes na compreensão da patogenia da doença multifatorial e na descoberta de alvos terapêuticos para novos abordagens terapêuticos.

Atualmente os focos da pesquisa são

Estudos de associação genéticas com variantes polimórficas funcionais selecionadas em componentes do inflamassoma em cortes de doenças multifatoriais (infecciosas ou não) e estudo do impacto funcional destas variantes.
Analise do inflamassoma em leucócitos humanos em modelos in vitro.
Testes funcionais para pacientes com doenças auto-inflamatórias monogênicas ou multifatoriais e sem caracterização genética.

Publicações recentes (2019-2021):

Recessive NLRC4‑Autoinflammatory Disease Reveals an Ulcerative Colitis Locus

Steiner A, Reygaerts T, Pontillo A, Ceccherini I, Moecking J, Moghaddas F, Davidson S, ·Caroli F, Grossi A, Fernandes Morato Castro F, Kalil J, ·. Goh FN, · Schmidt FI,· Bartok E,· Zillinger T, · Hartmann T Geyer T, Gattorno M, Oliveira Mendonça L, Masters SL.

Journal of Clinical Immunology 2021. doi: 10.1007/s10875-021-01175-4

Genetic contribution and functional impairment of inflammasome in sickle cell disease.

de Freitas Dutra V, Leal VNC, Fernandes FP, Souza CRL, Figueiredo MS, Pontillo A.

Cytokine. 2021 Oct 7;149:155717. doi: 10.1016/j.cyto.2021.155717.

NLRP1 acts as a negative regulator of Th17 cell programming in mice and humans with autoimmune diabetes.

Costa FRC, Leite JA, Rassi DM, da Silva JF, Elias-Oliveira J, Guimarães JB, Foss-Freitas MC, Câmara NOS, Pontillo A, Tostes RC, Silva JS, Carlos D.

Cell Rep. 2021 May 25;35(8):109176. doi: 10.1016/j.celrep.2021.109176.

Single-Nucleotide Variants in the AIM2 – Absent in Melanoma 2 Gene (rs1103577) Associated With Protection for Tuberculosis.

Figueira MBA, de Lima DS, Boechat AL, Filho MGDN, Antunes IA, Matsuda JDS, Ribeiro TRA, Felix LS, Gonçalves ASF, da Costa AG, Ramasawmy R, Pontillo A, Ogusku MM, Sadahiro A. Front Immunol. 2021 Apr 1;12:604975. doi: 10.3389/fimmu.2021.604975.

Common pathogen-associated molecular patterns induce the hyper-activation of NLRP3 inflammasome in circulating B lymphocytes of HIV-infected individuals.

Leal VNC, Reis EC, Fernandes FP, Soares JLDS, Oliveira IGC, Souza de Lima D, Lara AN, Lopes MH, Pontillo A.

AIDS. 2021 May 1;35(6):899-910. doi: 10.1097/QAD.0000000000002833.

Inflammatory effect of Bothropstoxin-I from Bothrops jararacussu venom mediated by NLRP3 inflammasome involves ATP and P2X7 receptor.

Ranéia E Silva PA, de Lima DS, Mesquita Luiz JP, Câmara NOS, Alves-Filho JCF, Pontillo A, Bortoluci KR, Faquim-Mauro EL.

Clin Sci (Lond). 2021 Mar 12;135(5):687-701. doi: 10.1042/CS20201419.

Sensing soluble uric acid by Naip1-Nlrp3 platform.

Braga TT, Davanso MR, Mendes D, de Souza TA, de Brito AF, Cruz MC, Hiyane MI, de Lima DS, Nunes V, de Fátima Giarola J, Souto DEP, Próchnicki T, Lauterbach M, Biscaia SMP, de Freitas RA, Curi R, Pontillo A, Latz E, Camara NOS.

Cell Death Dis. 2021 Feb 5;12(2):158. doi: 10.1038/s41419-021-03445-w.

Macrophage inflammatory state in Type 1 diabetes: triggered by NLRP3/iNOS pathway and attenuated by docosahexaenoic acid.

Davanso MR, Crisma AR, Braga TT, Masi LN, do Amaral CL, Leal VNC, de Lima DS, Patente TA, Barbuto JA, Corrêa-Giannella ML, Lauterbach M, Kolbe CC, Latz E, Camara NOS, Pontillo A, Curi R.

Clin Sci (Lond). 2021 Jan 15;135(1):19-34. doi: 10.1042/CS20201348.

Combining Host Genetics and Functional Analysis to Depict Inflammasome Contribution in Tuberculosis Susceptibility and Outcome in Endemic Areas.

Souza De Lima D, Bomfim CCB, Leal VNC, Reis EC, Soares JLS, Fernandes FP, Amaral EP, Loures FV, Ogusku MM, Lima MRD, Sadahiro A, Pontillo A.

Front Immunol. 2020 Oct 21;11:550624. doi: 10.3389/fimmu.2020.550624.

A Specific IL6 Polymorphic Genotype Modulates the Risk of Trypanosoma cruzi Parasitemia While IL18, IL17A, and IL1B Variant Profiles and HIV Infection Protect Against Cardiomyopathy in Chagas Disease.

Gomes Dos Santos A, Watanabe EH, Ferreira DT, Oliveira J, Nakanishi ÉS, Oliveira CS, Bocchi E, Novaes CTG, Cruz F, Carvalho NB, Sato PK, Yamashiro-Kanashiro EH, Pontillo A, de Freitas VLT, Onuchic LF, Shikanai-Yasuda MA.

Front Immunol. 2020 Oct 22;11:521409. doi: 10.3389/fimmu.2020.521409.

A case report of a novel compound heterozygous mutation in a Brazilian patient with deficiency of Interleukin-1 receptor antagonist (DIRA).

Mendonça LO, Grossi A, Caroli F, de Oliveira RA, Kalil J, Castro FFM, Pontillo A, Ceccherini I, Barros MAMT, Gattorno M.

Pediatr Rheumatol Online J. 2020 Aug 20;18(1):67. doi: 10.1186/s12969-020-00454-5.

Inflammasome genetics and complex diseases: a comprehensive review.

Fernandes FP, Leal VNC, Souza de Lima D, Reis EC, Pontillo A.

Eur J Hum Genet. 2020 Oct;28(10):1307-1321. doi: 10.1038/s41431-020-0631-y.

Inflammasome activation and IL-1 signaling during placental malaria induce poor pregnancy outcomes.

Reis AS, Barboza R, Murillo O, Barateiro A, Peixoto EPM, Lima FA, Gomes VM, Dombrowski JG, Leal VNC, Araujo F, Bandeira CL, Araujo RBD, Neres R, Souza RM, Costa FTM, Pontillo A, Bevilacqua E, Wrenger C, Wunderlich G, Palmisano G, Labriola L, Bortoluci KR, Penha-Gonçalves C, Gonçalves LA, Epiphanio S, Marinho CRF.

Sci Adv. 2020 Mar 4;6(10):eaax6346. doi: 10.1126/sciadv.aax6346.

Double-edged sword of inflammasome genetics in colorectal cancer prognosis.

Cambui RAG, do Espírito Santo GF, Fernandes FP, Leal VNC, Galera BB, Fávaro EGP, Rizzo LA, Elias RM, Pontillo A.

Clin Immunol. 2020 Apr;213:108373. doi: 10.1016/j.clim.2020.108373

Differential expression of the inflammasome complex genes in systemic lupus erythematosus.

da Cruz HLA, Cavalcanti CAJ, de Azêvedo Silva J, de Lima CAD, Fragoso TS, Barbosa AD, Dantas AT, de Ataíde Mariz H, Duarte ALBP, Pontillo A, Crovella S, Sandrin-Garcia P. Immunogenetics. 2020 May;72(4):217-224. doi: 10.1007/s00251-020-01158-6.

Transcriptional analysis of THP-1 cells infected with Leishmania infantum indicates no activation of the inflammasome platform.

Gatto M, Borim PA, Wolf IR, Fukuta da Cruz T, Ferreira Mota GA, Marques Braz AM, Casella Amorim B, Targino Valente G, de Assis Golim M, Venturini J, Araújo Junior JP, Pontillo A, Sartori A.

PLoS Negl Trop Dis. 2020 Jan 21;14(1):e0007949. doi: 10.1371/journal.pntd.0007949

Inflammasome in HIV infection: Lights and shadows.

Leal VNC, Reis EC, Pontillo A.

Mol Immunol. 2020 Feb;118:9-18. doi: 10.1016/j.molimm.2019.12.001. Review.

Enhanced expression of NLRP3 inflammasome components by monocytes of patients with pulmonary paracoccidioidomycosis is associated with smoking and intracellular hypoxemia.

Amorim BC, Pereira-Latini AC, Golim MA, Ruiz Júnior RL, Yoo HHB, Arruda MSP, Tavares AH, Cavalcante RS, Mendes RP, Pontillo A, Venturini J.

Microbes Infect. 2020 Apr;22(3):137-143. doi: 10.1016/j.micinf.2019.11.001

Antagonistic role of IL-1ß and NLRP3/IL-18 genetics in chronic HIV-1 infection.

Reis EC, Leal VNC, da Silva LT, Dos Reis MML, Argañaraz ER, Oshiro TM, Pontillo A.

Clin Immunol. 2019 Dec;209:108266. doi: 10.1016/j.clim.2019.108266.

Flagellin/NLRC4 Pathway Rescues NLRP3-Inflammasome Defect in Dendritic Cells From HIV-Infected Patients: Perspective for New Adjuvant in Immunocompromised Individuals.

Dos Reis EC, Leal VNC, Soares JLDS, Fernandes FP, Souza de Lima D, de Alencar BC, Pontillo A.

Front Immunol. 2019 Jun 11;10:1291. doi: 10.3389/fimmu.2019.01291.

Influence of NKG2C gene deletion and CCR5Δ32 in Pre-eclampsia-Approaching the effect of innate immune gene variants in pregnancy.

Kaminski VL, Ellwanger JH, Sandrim V, Pontillo A, Chies JAB.

Int J Immunogenet. 2019 Apr;46(2):82-87. doi: 10.1111/iji.12416.

Variants in NLRP3 and NLRC4 inflammasome associate with susceptibility and severity of multiple sclerosis.

Soares JL, Oliveira EM, Pontillo A.

Mult Scler Relat Disord. 2019 Apr;29:26-34. doi: 10.1016/j.msard.2019.01.023.