Organizations continually look for new ways to generate ideas and to convert them into innovative products and services, a movement that strengthens entrepreneurship. Considering the context of entrepreneurship education, this paper presents and analyzes the main results obtained with the realization of the “7600001 Innovation and Entrepreneurship” course, offered by the University of São Paulo Innovation Agency (AUSPIN), with support of the platform “Bota Pra Fazer”, an Endeavor and SEBRAE initiative. The paper promotes the discussion concerning best practices for the academic diffusion of entrepreneurship education considering the diffusion of the agile approaches (Design Thinking, Lean Startup and Business Model Canvas). It was possible to conclude that the experience with the 7600001 course, with a blended format and project-oriented learning, was adequate to improve the learning of entrepreneurship at the Brazilian academic environment according to the vision of the students involved.