Acoustography is a non-invasive quantitative ultrasonic technique that generates speckle-free images by leveraging the medium’s response to harmonic radiation force induced by ultrasound. Acoustography doesn’t rely on ionizing radiation. Instead, it uses ultrasound energy to produce near real-time images. Moreover, this technique can visualize features within materials that may be opaque or exhibit high absorption of electromagnetic waves. Acoustography capitalizes on the non-linear characteristics of acoustic propagation, where energy converts from high frequencies (typically MHz) to low frequencies (typically kHz). For example, for tissue characterization, ultrasound radiation force is used to excite tissues at a low frequency, and the corresponding acoustic response is then used to generate images, providing insight into the mechanical properties and potential abnormalities within the tissue. Biomedical applications of acoustography encompass the assessment of various organs such as the breast, prostate, arteries, liver, and thyroid, among others.